In exercise, correctly symmetric peaks are incredibly scarce. Within a chromatogram they often present some diploma of tailing. Peak tailing is measured with the tailing aspect T. This factor describes the height asymmetry, i.e. to which extent the shape is approximated into the beautifully symmetric Gaussian curve. The tailing variable is measured

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b) Ion exchange chromatography: On this HPLC variety, the separation takes place as a result of reversible exchange of useful teams. This theory is usually far better recognized by experiencing the posting ion exchange chromatography.Fig. three demonstrates an case in point wherein the yellow part has a powerful affinity with the mobile phase and m